| 1. | Bl21 . the dot blot hybridization verified that the scfv was secreted to the medium
从培养基中可检测到可溶性抗人绒毛膜促性腺激素的单链抗体。 |
| 2. | The analysis results of southern blot hybridization among all of specimens are coincidence with the detecting results of pcr products electrophoresis
所有标本进行southernblot杂交分析，结果予以证实。 |
| 3. | Northern blot hybridization showed that leacbf expression had the same tendency with the enzyme activity . and it showed that the transcription factor acbf maybe have some relation with the enzyme activity of pal
表明在紫外光照射条件下， leacbf编码的转录因子的变化趋势与苯丙氨酸解氨酶( pal )活性的变化趋势有一定的相关性。 |
| 4. | Northern blot hybridization showed that leacbf has well - distributed expression in different tissues of wild tomato ( pink stage ) . treatment the seedling with uv made the enzyme activity of the pal increased gradually and then decreased
用不同的紫外线照射时间处理番茄幼芽，苯丙氨酸解氨酶( pal )的活性呈先是增加，达到最大值后再减小的趋势。 |
| 5. | Northern blot hybridization showed that leerfl , leerf2 and pti4 ( a member of erebps in tomato ) expressed differentially in different parts of wild tomato fruit during ripening , which was related with fruit ripening
Northern杂交分析表明： leerf1 、 leerf2 、 pti4 (已发表的番茄erebps成员)基因在普通番茄果实的成熟过程及果实的不同部位都有差异表达，表现出与成熟进程的相关性。 |
| 6. | Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最后，用地高辛随机引物法标记成momp基因核酸探针，斑点杂交检测衣原体基因组dna ，灵敏度可达10pg ，且不能检出其它病原体的核酸。将核酸探针法与补体结合反应法对衣原体感染的诊断进行比较，初步证明该探针具有较高的敏感性与较强的特异性。 |
| 7. | Molecular examination and biological observation of transformed plants by growing resistant germinations we got 30 transformed plants . pcr and spot blotting hybridization analysis indicate that hsp70 anti - sense cdna had been integrated into tobacco genome . through observation and measurement we found 12 completely sterile , 9hyper - sterile , 3 partially sterile and 6 fertile and significant differences between them in stamen , height , and fruit weight
Pcr和点杂交分析表明， hsp70正、反义。 dna己经整合到烟草的基因组中。在进行田间生物学性状观察时， 30株转基因植株中有12株表现出完全不育， 9株高度不育， 3株部分不育和6株可育；不育植株在雄蕊、株高和果实重量等方面都存在明显的差异。 |
| 8. | Fingerprints of 5 strains of the inbred mice and 2 strains of the inbred rats was conducted using a nonisotopically hrp labeled jl - 02 by the second institute of the public safety bureau of china and southern blot hybridization , the author studied many fingerprints of the same dna , the different organic fingerprints of the same organism and fingerprints of parent and offspring . the patterns were completely different among the different strains and those of the samples from the same strain were completely identical
采用公安部二所自行研制的jl - 02多位点探针对5个品系的近交系小鼠和2个品系近交系大鼠进行了dna指纹分析，经过对同一dna的反复制作dna指纹图和同一个体不同组织进行的dna指纹图制作及对亲代和子代(同品系内和不同品系间杂交)间的dna指纹图比较。 |
| 9. | The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii . two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium . the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome . at the same time ; compared agrabactenum - mediated method with gene gun method , the transformation frequency of the former was 16 . 7 % , while the latter was 50 % , so gene gun transformation method was suitable for long ya liiliwn
用携带有几丁质酶基因和- 1 、 3葡聚糖酶基因的工程菌，通过农杆菌介导法和基因枪转化法转化龙牙百合，经pcr和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较，发现农杆菌介导法的转化率为16 . 7 ，基因枪法的转化率为50 ，因此可能基因枪转化法更适于龙牙百合的遗传转化。 |
| 10. | The more accurate localization of 3galt - l mrna expression in mouse brain as studied by in situ hybridization was in good agreement with the general expression pattern seen with northern blot hybridization . in newborn mice , the dense granular layer and the purkinje cell showed a strong signal
我们发现在小脑中1型链结构的变化与邵galt一1的表达变化基本一致:在新生鼠小脑中， 1型链结构在蒲肯野细胞和颗粒层细胞中都有表达，而在成年鼠小脑中， 1型链结构仅在蒲肯野细胞中表达。 |
